This quarter the South Florida sites were sampled and added to the Indian River and Ridge sites for ImageJ analysis. This data is being compiled and organized for analysis within the neural network software. The merging and comparing of the collected data is still in progress. No new data has been released since the last report since all of the data from the August sampling is still being organized and compiled. Hurricane Irma made landfall in South Florida on September 10, 2017 as a Category 3 storm on the Saffir-Simpson Hurricane Wind Scale. We are still gathering data from all of our sites and do not think that this storm will impair our ability to continue with the study. The sites in South Florida and Indian River region, are flatwood sites, and all experienced flooding of some kind, with a few still flooded weeks after the event. This will influence the health of these trees in the future. This study should offer a unique perspective on HLB status/severity and tree recovery after a hurricane/flooding event. More will be known about all of these locations when we visit all of the sites in November. Objective 1: Leaf nutrient thresholds Samples from the August sampling are still in the process of being analyzed. The August sampling included leaf sampling for ImageJ analysis, qPCR, leaf starch content, and nutrition, as well as tree canopy measurements, SPAD, canopy height and volume. All three locations were sampled around the same time in August and we should finally have one complete data set from all sites across all locations. This data will be added to our very large database for analysis using the neural network software Easy-NN. We will look at the August sampling date as a snapshot in time across all sites and locations for any possible connection or correlation with HLB severity. Objective 2: Determine soil conditions that favor root hair and VAM proliferation i. Soil sampling will be completed in November and we will have a large set of samples for further soil analysis that we would like to work on. We have completed the measurements of permanent wilting coefficient on the first two years of soil data. Soils from the South Florida area will be included into the data set in November and will be measured for all of variables the other two regions have been measured for, including organic matter content, and color analysis. ii. We are investigating a new system for root hair development using Valencia and Murcott seedlings in a nutrient solution. Test runs of seedlings exposed to nutrient fog and hydroponics is underway. Currently a preliminary trial of nutrient solution concentrations is underway for feasibility. Both the nutrient fog and the liquid hydroponics systems are currently working well. In the next quarter, we will choose a method an expand the greenhouse study to include the 3 nutrient solutions, 1. Complete fertilizer, 2. Complete fertilizer minus Phosphorus, with Rock Phosphate (RP) as the source of Phosphorus, and 3. Complete fertilizer minus Phosphorus, with Triple Calcium Phosphate (TCP) as the source of Phosphorus. Currently in the complete fertilizer trial run, we do see the presence of microscopic root hairs, which we hope to promote using the RP and TCP fertilizer solutions.