The project has three objectives: (1) Obtain mature tissues of the best transgenic lines. (2) Determine whether transgenics prevent psyllids from being infected. (3) Continue testing generations of vegetative propagation from the best transgenic lines. The following work has been conducted in this quarter: (1) Analyzed another batch of rootstock transgenic lines (54 Carrizo lines, 7 Swingle lines, and 3 C-MAC lines). Two more Swingle lines and three C-MAC lines accumulated high levels of transgene products. More than half of the Carrizo lines accumulated high levels of transgene products. These rootstock transgenic lines will be analyzed based on the protein levels. (2) Conducted another cage experiment for replicates of the transgenic lines with low numbers of psyllid progenies. (3) Made more replicates of the transgenic lines tolerant to HLB.