17-002C Support for the Southern Gardens Diagnostic Laboratory

17-002C Support for the Southern Gardens Diagnostic Laboratory

Report Date: 04/17/2019
Project: 17-002C   Year: 2019
Category: CLas Bacteria
Author: Michael Ireys
Sponsor: Citrus Research and Development Foundation

This project is a continuation of funding that has been provided to Southern Gardens Citrus (SGC) to provide growers and researchers with a facility to do testing to detect�Candidatus Liberibacter asiaticus, the causal agent of citrus greening in Florida.� This report covers the third quarter of year two funding.� For the period of January 1, 2019 to March 31, 2019, a total of 6,951 samples were processed and tested by qPCR.� Of these 95% were plant samples and 5% were psyllid samples.� Virtually all of the plant samples were from grower, private entity, or grower research trials.� To date, for the two-year project, a total of 42,466 samples have been processed and tested.� Based on the current trends, it is expected that the total number of samples that will be processed during the grant period will be approximately 50,000 (budgeted amount was 60,000).� If the lab sample load does not reach the budgeted amount, the final bill will be adjusted as necessary to reflect the total number of samples actually run.�One trend that is changing is that more customers are requesting copy number information instead of just a positive/negative determination.� It is expected that approximately 50% of the year two samples were be provided to customers with copy number determination.� In addition, requests are coming in to provide testing using different primer sets and to return the DNA extracts back to the customers for additional in-house or custom testing.� When possible, the SGC lab has tried to accomodate these requests.


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