The objective of this is to use “new technologies” to accelerate the elimination of graft transmissible pathogens in germplasm accessions for use in citrus breeding in Florida. These “new technologies” include the application of cryotherapy (freezing the buds in liquid nitrogen followed by recovery of the treated buds by grafting onto seedling rootstocks) and the use of “mini-plant-indexing” which allows the biological indexing for graft transmissible pathogens using young seedling indicator plants, 60-75 days old seedlings. During the reporting period advanced citrus selections were passed through cryo treatment. Shoot tips of each selection have been recovered following treatment to determine viability. CLas-infected bud eyes were passed through cryo-treatment in the previous rating period. Plants recovered from these shoot tips are to be transfered to the USHRL where they will be tested for CLas and observed for development of HLB symptoms. Seven promising USDA promising new scion selections were propagated and readied for shipment to Ft. Collins. These selections were passed through cryo treatment and are in cryogenic storage. During the next quarter plants will be regenerated from cryo treated tissue to determine recovery and estimate % of viable shoots tips in storage. Regular shipments of additional selections from the USHRL to Ft. Collins will continue throughout the remaining duration of this project.