1. Please state project objectives and what work was done this quarter to address them: The objectives of this project are to produce disease resistant, commercially & agronomically desirable, mature citrus transgenics, intragenics, & non-GMO gene edited trees using Agrobacterium & biolistics as a service for research & commercialization. The research focus of this project is to improve transformation efficiencies, so that the mature citrus protocols become more productive, decrease prices for scientists, & contribute more to financial self-sufficiency of our lab. Our facility received an order from California to overexpress a citrus peptide in Hamlin, Kuharske & US942 using biolistic transformation to produce intragenics (all citrus sequences). The plants produced using this technology are not regulated & can be provided to the growers rapidly after field testing. We have started co-bombardments in these cultivars using other vectors until we receive the peptide. If this technology is successful, I will contact other scientists who might be interested in plant improvement using this method. For another scientist, 19 intragenics were produced using Agrobacterium transformation of mature EV1 scions. Twenty-four flowering shoots were produced & micrografted onto rootstock for another scientist. This quarter, a total of 52 transgenic or intragenic shoots were produced in EV1 or Kuharske and micrografted for different scientists. RNAi was also used to silence genes in another transformation experiment & a manuscript was published. A manuscript describing a protocol to increase transformation efficiency in mature citrus was submitted for review. Another manuscript will be submitted soon about a new intragenic selectable marker. 2. Please state what work is anticipated for next quarter: Co-bombardments to overexpress the citrus peptide will continue. We must finish some orders & new orders for Valencia, Early Valencia I, & Kuharske are pending. We just completed an experiment with Agrobacterium mediated gene editing & the results are pending. 3. Please state budget status (underspend or overspend, and why): CRDF funding is sufficient, but my EBA has been revised to better fund salaries. I am not sure how long it will take to move through the UF system. In the interim, Dr. Rogers funds the rest of our salaries.