The objectives of this project are to produce mature citrus transgenics that will flower & fruit naturally using Agrobacterium as a service for customers, increase transformation efficiency, & conduct research to further biolistic transformation, so that it will also become an efficient service requiring less deregulation. The number of transgenics produced, successfully micrografted, & secondary grafted for this quarter are listed in Table 1 below: Table 1. The number of transgenics produced for customersCustomers Vectors Cultivars # Transgenics Zale A Kuharski 5 Dutt B B770 1 Dutt C Kuharski 2 Mou D Hamlin 5 Mou E Kuharski 10 Mou E Swingle 1 Mou E X639 1 Dutt F US942 3 Dutt G Kuharski 11 Dutt H Valquarius 1 Total 40 Five transgenics must still be verified with PCR because the transgene rearranges in 30% of events. In addition, not all transgenics have been transferred to customers because they were too small at the time of UF closure because of the global pandemic, so we are maintaining them in cone-tainers. Five transgenics out of ~25 with the new plant selectable marker were micrografted for the Zale lab & have been transferred to the growth room to encourage growth for molecular analyses.