Grove selection is completed and in some areas, two cycles of data have been collected. The Indian River area is just completing their first round of sampling, while the south Florida and Ridge area have completed two rounds. Objective 1: Leaf nutrient thresholds In the Indian River locations, canopy measurements were taken and leaves were collected for starch, PCR and nutrition, as well as soil sampling for color, nutrients, and organic matter content. In the other two regions leaf sampling was completed to monitor leaf nutrient analysis, and canopy measurements were taken to track the growth for the study areas. The lab responsible for the PCR and starch analysis should be working on the samples in the next quarter. Objective 2: Determine soil conditions that favor root hair and VAM proliferation i. As mentioned in Objective 1 above, initial soil sampling at the 0-6 inch and 6-12 inch depth has been completed now at the Indian River location. Percent OM, using Loss on Ignition has been completed for the Ridge and Indian River locations and in the next quarter the initial samples from the remainder of the survey areas will be analyzed. Analysis includes, soil nutrient content, pH, CEC, and soil color. ii. The cuttings that were placed in aeroponics tanks are continuing to be monitored and maintained with replacement solutions bi-weekly. The solution chemistries are stable at this point; however, the growth of the cuttings has been slow. Analysis of the root development will be the next step of this portion of the study.