In November and Early December, all sites across all three regions were visited and sampled. Analysis is in progress of the latest soil samples. This data is being compiled and organized for analysis within the neural network software. The merging and comparing of the collected data is still in progress. No new data has been released since the last report since all of the data from the November sampling is still being analyzed, organized and complied. Objective 1: Leaf nutrient thresholds Samples from the November sampling are still in the process of being analyzed. The November sampling included leaf sampling for ImageJ analysis, and nutrition, as well as tree canopy measurements, SPAD, canopy height and volume. All three locations were sampled around the same time in either late November or Early December adding further to our tri-area data set from all sites across all locations. These data will be added to our comprehensive database for analysis using the neural network software Easy-NN. We will look at the November sampling date as a snapshot in time across all sites and locations for any possible connection or correlation with HLB severity. Objective 2: Determine soil conditions that favor root hair and VAM proliferation i. Soil sampling was completed in November and we will have a large set of samples for further soil analysis that we continue to work on. We have completed the measurements of permanent wilting point on the first two years of soil data. Soils from the South Florida area will be included into the data set in November and will be measured for all of the variables the other two regions have been measured for, including organic matter content, and color analysis. ii. We have selected the liquid nutrient solution over the fog hydroponic system for the seedling study using Murcott seedlings. Currently we have 3 tanks running with 9 Murcott seedlings each. We are utilizing three nutrient solutions, 1. Complete fertilizer, 2. Complete fertilizer minus Phosphorus, with Rock Phosphate (RP) as the source of Phosphorus, and 3. Complete fertilizer minus Phosphorus, with Triple Calcium Phosphate (TCP) as the source of Phosphorus. Our next step in this study is to analyze the roots for any root hair development, and make minor changes to the phosphorus/calcium amounts to accelerate root hair development.