Juvenile Tissue Citrus Transformation Facility (JTCTF) stayed open during the third quarter of 2021. However, the facility was idled for two weeks in August due to loss of employees and throughout that period did not perform any experiments. Within the last three months, JTCTF received five orders. None of these orders are associated with research grants and will be paid for. The work on these orders is starting. Requests for transgenic Valencia plants represented high majority and came at the time when JTCTF had in stock some Valencia fruits harvested earlier this year. These fruits started rotting in storage and extracted seeds had internal pathogens invisible to naked eye. This lead to contamination of starting material for our experiments, waste of lab resources, and slowdown in satisfaction of orders. For one of the orders, we were provided contaminated Agrobacterium culture which also resulted in contamination and disposal of plant material in multiple experiments. One of the orders was placed for transgenic Citrus macrophylla plants that are extremely difficult to produce and we are in the process of optimizing this process. For two orders placed earlier this year, we were not able to produce transgenic shoots and in consultation with the client the work with Valencia oranges was suspended and will begin with Duncan grapefruit. At the end of July, the work on multiple orders associated with the USDA grant where I am still an active participant stopped because of departure of employee who worked on them. Newly appointed person is expected to continue this work in October. For the initial phase of its existence as an EBA, JTCTF is envisioned to operate having two employees and a manager. One employee is full-time employee and the other is part time employee working three days a week. This labor force should be able to process 15-20 orders per year. In the month of September, JTCTF operated in this mode but was slowed down because of above-described circumstances. Because of the period of idling as well as time lost because of the contamination of experimental material, JTCTF has had very low productivity in the third quarter. Only six transgenic plants were produced including four Duncan grapefruit plants and two Valencia plants. What also contributed to this outcome is that for multiple earlier orders associated with the USDA grant, JTCTF produced transgenic shoots that were neither grafted nor rooted into plants. Results from these experiments served as a basis for design of new experiments. The Business office staff at CREC continued to provide assistance in processing of payments for the services JTCTF offers.