1. Please state project objectives and what work was done this quarter to address them: Objective 1. Investigate effects of rootstock propagation method and the interaction with rootstock on root structure, root growth, and tree performance during the first 3 years of growth in the field.A manuscript with the detailed findings of trial 1 has been accepted for publication in the journal HortScience and can be accessed at: https://doi.org/10.21273/HORTSCI15507-20.Bimonthly root growth measurements with the minirhizotron imaging system continued in trial 2 (Hendry County) and trial 3 (Polk County). The root core data analysis to assess fibrous root traits was completed.DNA extraction and CLas detection by PCR was completed.Leaf nutrient results were obtained and data analysis was completed. Data are currently assembled into one manuscript to be published later this year. Objective 2. Investigate if trees on rootstocks propagated by tissue culture or cuttings differ in susceptibility to Phytophthora-induced decline or wind-induced blow-over compared with trees on rootstocks propagated by seed.Monthly root growth measurements with the rhizotron imaging system continued.Tee horticultural measurements were conducted. Leaves and roots were collected for pathogen analysis. Leaves were collected for nutrient analysis. 2. Please state what work is anticipated for next quarter: We will continue with our minirhizotron root imaging. We will continue with the statistical analysis and interpretation of all data collected. We will start processing tissue samples for PCR/ELISA analysis. 3. Please state budget status (underspend or overspend, and why): Approximately 56% of funds have been spent which is slightly underspent due to Covid-19 related complications that affected research, travel, and hiring of personnel.