Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 08/01/2024
Project: 23-029   Year: 2024
Category: Plant Improvement
Author: John Chater
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them: Objective 1: Destroy declining plant material with no direct use to the citrus industry to make space for Stage I and Stage II trials (Years 1 and 2).Since project planning and the beginning of the project period, several thousand citrus trees have been pushed and destroyed at CREC, a portion of which is for making space for Stage I and Stage II trials.  Of these several thousand trees, approximately thousands are slated to be pushed and destroyed since the beginning of the last reporting period.  Hundreds of trees to up to 1,000 have been identified as being suitable for conservation, movement with the tree spade (or other means) and sourced for budwood.  Trees were identified by both drone and by visual inspection.  The nursery is aware of the need to collect and propagate uncertified budwood and they have given the go-ahead to transfer budwood immediately.  UFarm charges have conserved and saved all materials needed for data mining for tolerant genes and other variants. Objective 2: Conserve, move, consolidate, and propagate tolerant and resistant citrus trees to a centralized location. (Years 1 and 2).The block where consolidation will take place has been reserved for this purpose.  The nursery will receive the first budwood on Tuesday 8.6.24.  When the buds take, we will move forward with tree movement with tree spade and other means.  We do not have possession of the tree spade.  The tree spade has been repaired and we are waiting for the UF/IFAS payment to process before bringing it to the CREC to begin practicing moving trees of the appropriate size.  2. Please state what work is anticipated for next quarter: We anticipate using the USDA-ARS tree spade to move the first trees and transferring budwood to the nursery for propagation, which will start on this coming Tuesday.  1,000 budeyes from 84 accessions are planning to be transferred to the nursery.  We also anticipate removing thousands of additional citrus trees with no immediate value to the citrus industry to continue to make space for Stage I and II trials as well as breeding populations.    3. Please state budget status (underspend or overspend, and why): We are no longer underspent (29% time spent versus 40% funded expended) according to the UF/IFAS grants tracking system. The budget is being expended at a rate at which all funding will be expended by the end of the project period.   The payment has been made to the contractor, and we are on track for spending.    


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