Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 04/19/2024
Project: 23-029   Year: 2024
Category: Plant Improvement
Author: John Chater
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them: Objective 1: Destroy declining plant material with no direct use to the citrus industry to make space for Stage I and Stage II trials (Years 1 and 2).Since project planning and the beginning of the project period, several thousand citrus trees have been pushed and destroyed at CREC, a portion of which is for making space for Stage I and Stage II trials.  Of these several thousand trees, approximately 1,000 trees were pushed and destroyed since the beginning of the project period.  Hundreds of trees have been identified as being suitable for conservation, movement with the tree spade (or other means) and sourced for budwood.  The nursery is aware of the need to collect and propagate uncertified budwood and we are waiting on the go-ahead from the nurserymen and -women to transfer budwood of the trees identified for conservation and consolidation to the nursery.  The drone project has identified trees that should be targeted for consolidation and ground truthing these trees has been completed at two large blocks of Plant Improvement land.  UFarm charges have conserved and saved all materials needed for data mining for tolerant genes and other variants. Objective 2: Conserve, move, consolidate, and propagate tolerant and resistant citrus trees to a centralized location. (Years 1 and 2).The block where consolidation will take place has been reserved for this purpose.  We are awaiting the nursery’s go-ahead to propagate the material.  Rootstocks have been selected.  When the buds take, we will move forward with tree movement with tree spade and other means.  We do not have possession of the tree spade.  According to the contractor who is repairing it, the tree spade will be ready in two to three weeks. We are developing contingency plans if the nursery delays the budwood transfer.  2. Please state what work is anticipated for next quarter: We anticipate taking possession of the USDA-ARS tree spade from the contractor and transferring budwood to the nursery for propagation.  We also anticipate removing thousands of citrus trees with no immediate value to the citrus industry to continue to make space for Stage I and II trials.    3. Please state budget status (underspend or overspend, and why): We are 1% underspent (15% time spent versus 14% funded expended) according to the UF/IFAS grants tracking system. The budget is underspent as we wait for the repairs of the tree spade.  Once that payment is made to the contractor, we should be on track for spending.  It was not anticipated for the tree spade repair to take more than three months.    


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