Contined funding for the mature citrus facility to produce disease tolerant, transgenic citrus.

Contined funding for the mature citrus facility to produce disease tolerant, transgenic citrus.

Report Date: 10/07/2015
Project: 15-045C   Year: 2015
Category: Horticultural & Management
Author: Janice Zale
Sponsor: Citrus Research and Development Foundation

Mature plant production continues with various genetic constructs with reporter genes from Drs. Dutt, McNellis and Wang. Additional scientists have expressed interest in our services. Transformation efficiencies have significantly increased with reporter genes. We are also trying to further increase Agrobacterium transformation efficiencies of mature citrus by incorporating vacuum infiltration and sonication treatments. These treatments significantly increased transformation efficiencies in immature citrus. In addition to Agrobacterium, we can now supplement plant production with plants produced using biolistics. Scions and rootstocks have been micropropagated (budded scions and rooted cuttings) into three replicates for one location. It remains to be determined whether we will continue micropropagation for replicates at additional locations. We have almost concluded screening Dr. Orbovic’s putative transgenics with qPCR to identify high-expressing lines. This work should be finished by the end of this month. I am switching to citrus pots in the growth room, which can be planted to higher densities than current planting densities. Recently we discovered that mature scion grows significantly faster after budding if the rootstock is not cut-off after the buds break.


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