Our productivity significantly decreased after the move to the packing house while the AC in our lab was being fixed. There was bacterial contamination of our cultures, presumably due to autoclave issues, unsealed windows, or poor temperature control. Bacterial and fungal clean tests of mature citrus budwood from the growth facility in LB & LW broth, respectively, showed that all mother trees were clean, even the new cultivar introductions (B770, OLL8, Vernia, red grapefruit). We anticipate having to do two Agrobacterium transformations per week to make-up for lost time. Needless to say our efficiencies declined because of the move. Due to the aforementioned difficulties, Agrobacterium transformations with disease resistant genes was slowed. Only ~10 transgenics were produced and one did not survive micrografting. The results of the remainder are pending. Ten immature Swingle transgenics for Dr. Wang and Vladimir were micrografted because Vladimir had micrografting issues in the packing house. One shoot died & the results for the others are pending. We have found a cDNA that dramatically increases our mature scion transformation efficiencies and we are investigating whether it will increase efficiencies in all cultivars. An invention disclosure entitled, A method to increase organogenesis and transformation efficiencies in recalcitrant woody species such as mature citrus, was submitted to UF/IFAS Tech Transfer. There have been significant unanticipated growth room repair & maintenance expenditures during this last quarter. The water softener had to be rebuilt & a new one must be purchased next fiscal year. Without the water softener, hard water clogs the humidifiers & a white residue is deposited on plants making them unsuitable for transformation. The AC ducts in both growth rooms must be replaced because of filth deposited inside them over the years. The sprayer broke down & was repaired again. In the future, a new sprayer must be purchased to alleviate costly repairs. Lights & ballasts are an ongoing significant expenditure. We had to replace some of the shelving in the laboratory because our shelving disappeared after the move. The use of the PMI selectable marker after biolistics of immature and mature citrus continues. Different sucrose concentrations are required for shoot regeneration in mature vs immature citrus. Similarly, more sucrose is necessary for shoot development in scion than rootstock. Mannose concentrations must be manipulated accordingly. A manuscript (25% funded CRDF & 75% funded CRB) was submitted to PCTOC & is in review: Y. Acanda, M. Canton, H. Wu and J. Zale (XXXX) Kanamycin selection in bioreactors allows visual selection of transgenic citrus shoots, PCTOC.