Report for period ending 12/2016 During this quarter we surveyed ACP populations across the state to compare the wild populations of ACP with our laboratory colony in terms of amount of the three neonics required to control ACP based on method of delivery. Sites chosen to collect psyllids included locations in Vero Beach, Lake Placid, Lake Alfred and LaBelle FL. In all cases, the wild psyllid populations responded in a similar manner to our laboratory colony with the LC50 and LC90s for ingestion being far greater than those values for the contact assays. We also did note some variation in the amount of product required to reach an LC90 value. This results suggests that more monitoring should be conducted for potential shifts in psyllid susceptibility to the nenicotinoid insecticides. We have also been making great progress with our EPG studies of psyllid feeding on artificial diets to determine the amount that is needed to cause psyllids to quit feeding (not necessarily die) and thus reduce transmission probabilities. Here we have been able to identify the waveforms produced that are drastically different from those in plants and then begin record and analyze psyllid feeding on artifical diets containing varying levels of iinsecticides to develop the LC50/90 data for mouthpart withdrawal. As previously reported…we are continuing to analyze the large backlog of leaf samples gathered from our ongoing field studies where we are investigating the uptake of the three neonics at different times of the year, distribution of the three neonics within a tree, and appropriate rate of product applied based on tree size. We literally have thousands of samples in the freezer awaiting analysis. We continue to run samples as fast as the machine can analyze them.