Create citrus varieties resistant or tolerant to Huanglongbing through transgenic and nontransgenic approaches

Create citrus varieties resistant or tolerant to Huanglongbing through transgenic and nontransgenic approaches

Report Date: 05/04/2017
Project: 15-020   Year: 2018
Category: Horticultural & Management
Author: Zhonglin Mou
Sponsor: Citrus Research and Development Foundation

The project has three objectives: (1) Obtain mature tissues of the best transgenic lines. (2) Determine whether transgenics prevent psyllids from being infected. (3) Continue testing generations of vegetative propagation from the best transgenic lines. The following work has been conducted in this quarter: (1) Conducted two more rounds of cage experiments to further test if the transgenic plants inhibit psyllid reproduction. Results showed that none of the transgenes was able to significantly reduce psyllid progeny numbers in the cage experiment. (2) Newly generated replicates of the transgenic lines were inoculated in the psyllid room and nymph-infested plants were recorded and moved to the immediate greenhouse for symptom development.


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