Create citrus varieties resistant or tolerant to Huanglongbing through transgenic and nontransgenic approaches

Create citrus varieties resistant or tolerant to Huanglongbing through transgenic and nontransgenic approaches

Report Date: 11/16/2016
Project: 15-020   Year: 2016
Category: Horticultural & Management
Author: Zhonglin Mou
Sponsor: Citrus Research and Development Foundation

The project has three objectives: (1) Confirm HLB resistance/tolerance in transgenic citrus lines. (2) Determine the chimerism of the HLB-resistant/tolerant transgenic lines. (3) Confirm HLB resistance in citrus putative mutants (nontransgenic lines). The following work has been carried out in this quarter: (1) Inoculated the promising candidate transgenic plants with CTV carrying the flower-promoting gene FT3. (2) Propagated the transgenic line HAM 13-3, DUN 57-25, DUN205-25c, and DUN 207-8. (3) Infected progenies of transgenic plants with Las-carrying psyllids. We have generated more transgenic plants expressing various defense genes. These transgenic plants are growing in greenhouse and will be tested once they are ready.


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