1. Please state project objectives and what work was done this quarter to address them: Induce efficient flowering in citrus seedlings by overexpressing FT3 and knocking out negative regulators of flowering. Work done during this quarter:As N. benthamiana infected with the different constructs expressing FT3 revealed systemic symptoms, we concentrated virions and inoculated into citrus. Further, we did RT-PCR stability assays and sequencing for three of vectors from the systemic tissue of N. benthamiana. RT-PCR assays revealed a single band of the expected size that was isolated and send for sequencing. Sequencing analysis revealed 100% homology when compared to theory sequence. We expect a flowering phenotype from some of those vectors infected to citrus with the spring flush.Among the transgenic carrizo plants there is segregation with some plants flowering whereas others coming from the same line are not flowering. 2. Please state what work is anticipated for next quarter: In the next quarter, we will do sequence analysis from the different CTV FT3 vectors infected into citrus upon becoming positive by ELISA. Further, we will infect transgenic FT3 lines with CTV-RNAi vectors targeting negative regulators of flowering. 3. Please state budget status (underspend or overspend, and why): On budget