1. Please state project objectives and what work was done this quarter to address them:The objectives of this project are: 1, To study the effect of Brs on priming immunity on young, newly planted trees. This will allow to know for how long immune response will last after Br application, so we can adjust timing (number of applications). We planted 40 Valencia trees on carrizo and 40 trees on X639 in a complete randomized block design. Br application or water (as control)has been performed every 15 days. We have been monitoring psyllid infestation and collecting leaves for bacteria titer analysis.Tree growth is being monitored. It is still early to have any significant data, but psyllids are clearly less abundant in Br-treated plants. To better undesrtand the effects, we started an experiment before August 1st in controlled conditions in the greenhouse with the same setup of trees, that were enclosed in individual cages and 4 pairs of psyllids per cage were introduced. We have seen differences in oviposition and activation of SAR genes with treatment and we found that this activation lasts for at least 6 weeks. This opens the possibility of reduce the number of Br applications. We are still monitoring this in the open field.2, To determine the best time of application (frequency) to achieve maximum protection against pests and disease in newly planted trees. This objective is related to the first one. In discussions with some growers at our Citrus advisory committee, they raised the posibility of concentrating the Br application when psyllids are more abundant, durnig or at the beginning of flushes. I think this idea has good potential, so we are going to assay this as well. 3, To determine the effect of Br application on advancing fruit maturation in both Valencia and Hamlin. We started Br applications at Duda farm by the end of August in Hamlin. Treatments have been performed since then twice per month. The treatments are being performed in commercial conditions. While we did not see a statistically significant increase in Brix in September, during October we have started to see a slight increase: 8,6 as compared to 8,1 in average. Interestingly, acidity has been consistently lower in fruit from Br-treated trees, making the ratio significantly higher. In view of this fact, we have started to collect juice samples for further HPLC analysis of sugars and organic acids. Fruit drop has started for Hamlin and we are monitoring it weekly. Unfortunately, hurricane Ian has accelerated fruit drop and we have also lost some trees. However, our close monitoring of fruit drop, that we started before Ian is allowing us to actually assess the real impact of the hurricane on fruit drop. Outreach:-Invited talk. Brassinosteroids and fruit quality. Packinghouse day. August 27, 2022, Lake Alfred FL.-Invited talk. Combining IPCs and Brs to prolong young tree health. Updates and future steps. Citrus Expo 2022, August 18, Fort Myers. 2. Please state what work is anticipated for next quarter: For objective 3, we will harvest Hamlin when the commercial time is right, we will assess yield and final quality. We will also start in December treatments in Valencia.For objectives 1 and 2, treatments and samplings will continue as in this quarter. 3. Please state budget status (underspend or overspend, and why):Spending is on track. Student is on board and we will start spending more this quarter as we start in depth molecular analysis