A Ca. L. asiaticus medium, AM, was proposed based on the components of a defined medium for L. crescens plus the addition of some compounds found on the phloem biochemical composition of citrus. So far, we have developed 5 modifications to AM, being AM2 the most promising one. Different inoculations procedures have been done using either a mix of psyllids guts or spin guts supernatant into the different media. Traditional PCR results indicated the presence of L. asiaticus inoculum in the cultures after three weeks; however, not turbidity associated with growth has been seen to date. We are currently exploring different possibilities such as qPCR and sequencing to verify that L. asiaticus is actually growing in the media. With collaboration of Dr. Killiny�s lab, we are currently analyzing other important components within the phloem sap of citrus to be added to AM2. We expect to propose new formulations based on this information.