1. Please state project objectives and what work was done this quarter to address them:Objective 1: Document laboratory and field biology- reproduction, development time, where snails lay eggs, periods of life stage activityField: We have continued the biweekly monitoring of snail populations in central Florida and will continue this for a minimum of 2 years total. In this reporting period, we document a period dominated by inactivity (January-February) and the beginning of field activity in central Florida in early March, with more consistent activity later in March. Laboratory: The Quinn lab has initiated an experiment to determine the temperature-dependent development of B. bonariensis. This is important for understanding when the snails’ eggs are likely to hatch and subsequently impact citrus and other crops. Freshly-laid snail eggs will be held in environmental chambers set to a range of temperatures and checked daily for whether or not the eggs have hatched. This experiment will be ongoing throughout the year. Objective 2: Determine factors that influence snail movement/dispersal While not an expected factor, we have noted that in cooler temperatures, these snails burrow into the soil/sand substrate and do not return to regular activity until ambient temperatures increase. This activity may be something we can take advantage of and we will explore more in future quarters. Quinn lab is also evaluating potential deterrents to the snails with a goal of finding an odorant that could be reduce snail attraction to specific items, such as emitters. Of the materials tested, Copper II Sulfate and Deet show promise so far. Objective 3: Evaluation of baits/insecticidesNo work could be completed this past quarter due to the above noted burrowing behavior of the snails. Objective 4: Evaluation of predatorsNo work could be completed this past quartr due to the above noted burrowing behavior of the snails. 2. Please state what work is anticipated for next quarter:Obj 1: Field and lab studies will continue this quarter as in previous. Obj. 2: Deterrent assays will continue this quarter. Obj. 3: Evaluation of pesticides and baits will continue throughout this quarter. We have a list of pesticides, including insecticides, herbicides, and fungicides, to test for activity against the snails. Obj. 4: We are prepared to begin deploying camera traps this spring. This will allow us to visually determine the potential predators of the snails present in citrus groves. We are also planning to collect snails to determine what plants they are eating (citrus, weeds, etc.) via molecular gut content analysis, which will be important for pest and habitat management. We will also collect potential natural enemies (generalist predators) in citrus groves and analyze their gut contents to determine whether or not they have eaten the snails. This molecular information will reveal the ecological relationships of B. bonariensis and other organisms in citrus groves, which could lead to improved pest management strategies. 3. Please state budget status (underspend or overspend, and why):We are currently on track to be out of money before our 6 month NCE is completed. It appears that the REC for our collaborator did not charge the grant in a timely fashion, making in appear as though we had more funds available in December than we truly did. 4. Please show all potential commercialization products resulting from this research, and the status of each: none yet