1. Please state project objectives and what work was done this quarter to address them: (1a) Field monitoring: Redesigned traps evaluated and will work for population monitoring. Pheromone attraction protocol has been designed and works in a laboratory setting. (1b) Laboratory screening of conventional insecticides and entomopathenogenic fungi has been completed and accepted for publication. Adjuvant screening started in December 2020. (1d) Ant management: Preliminary data shows a greater abundance of known and potential predators associated with the mealybugs when ants were prevented from accessing the bugs, the most common ant collected in both groves was the red imported fire ant. A field trial for removal of fire ants was prepared and ant density was evaluated, trial had to be delayed due to key collaborator getting COVID. (1e) Entomopathenogenic Fungi (EPF) field tests for use in IPCs was completed with the final samples being evaluated presently. Preliminary data suggest that EPFs may provide 3-4 weeks of control when deployed in IPCs. (2a) Assessment of predators for mealybugs: two years of field data have been collected to determine what predators are present in two locations, samples are still being sorted and identified. MS student K. Gaines created a DNA primer to enable evaluation of gut contents for lebbeck mealybug. This DNA primer has been fully vetted and works well in our control trials. Predator screening assays for potential predators that could be added to the system from what is commercially available for release has been started). (2c) EPG library for mealybug feeding has been started and we are making progress. This information will be necessary to evaluate chemistries that may inhibit feeding. (2d) Minimize spread: We have resumed working on developing recommendations to reduce spread, with a focus on reducing likelihood of accidental movement on individuals. 2. Please state what work is anticipated for next quarter: (1a) Mealybug population monitoring to begin January 2021. Pheromone trapping with live females and new lures from a collaborator will be tested in groves. (1b) Soil drench materials will be evaluated under laboratory conditions for prevention of infestation starting in late January/early February 2021. (1c) Evaluate promising materials in open grove setting: will deployed in March/April. (1d) Ant management: trial is scheduled to be deployed in February 2021. (1e) Management options for IPCs: field plots will be infested in late January 2021 for clean-up testing once populations have established. (2a) Molecular marker for lebbeck mealybug presence in guts of field collected predators will be started. Predator assays for potential mealybug predators to be released will continue. We plan to perform a field survey for other predators using methods used for Pink Hibiscus Mealybug and intend to begin this in the spring or early summer. (2c) We will continue working on the EPG library to enable the feeding inhibition trials to occur. (2d) Minimize spread: We will complete the testing of materials to reduce spread on people and resume steam sanitation work. 3. Please state budget status (underspend or overspend, and why): We are back on track with budget spending as we were able to resume focus on this project once COVID protocols were in place