Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Report Date: 08/15/2023
Project: 21-008   Year: 2023
Category: Plant Improvement
Author: Kim Bowman
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:Objective 1. Collect field performance data from replicated rootstock field trials and release new rootstock cultivars as justified by superior performance in multiyear field trials.Collect field performance data.  Thirty-one USDA replicated rootstock trials are active.  For the current quarter, canopy health and tree size data was collected from all trials.  Juice analysis continued from trials for the 2022-23 season.  Some problems developed with the autotitrator and spectrophotometer used for the fruit quality analysis which slowed down the juice analysis.  New equipment is being purchased with USDA funding to provide higher throughput analysis of juice samples beginning in the 2023-24 season.During this quarter, SSR assessment of trueness-to-type from seed was completed for the SuperSour rootstocks that are available now and will be released this year.  This information is being prepared for publication that will coincide with the official release of the new rootstocks.  Additional work on trueness-to-type assessment and salinity tolerance of new rootstocks was continued.Preparation for release. The most promising USDA rootstock selections are provided to the FDACS-DPI program for shoot tip grafting and disease testing.  The two new USDA rootstocks planned for release this year are already clean and available in the DPI program.  Four other promising new USDA rootstocks were provided to DPI for STG and testing this quarter, in preparation for possible release in future years.Release of new rootstocks. New rootstocks will be officially released by USDA for commercial use when justified by superior performance in multi-year replicated field trials.  The rootstocks US SuperSour 4 and US SuperSour 5 have moved to the final stage of approval for release by USDA.Objective 2. Create hybrid rootstocks that combine germplasm from parental material with good rootstock traits and HLB tolerance, propagate the most promising of these hybrids, and establish replicated field trials with commercial scions.Create hybrids. Selected new crosses were completed in spring 2023, and good fruit set was achieved.  Fruit will be monitored and harvested for seed extraction later in 2023.Propagate hybrids. Budded trees continued to be grown in the nursery for a rootstock trial with a new high quality scion, with field planting scheduled for August.  Trees for two new stage 2 rootstock trials with thirty SuperSour rootstocks were budded with Valencia in this quarte, with a plan for field planting in 2024.Establish replicated field trials. One new replicated field trial with a new high quality scion on 14 rootstocks was established with a commercial cooperator this quarter.Tree care in trials. Periodic care was applied in the 31 field trials to maintain tree health and productivity, and manage weeds.2. Please state what work is anticipated for next quarter:For the coming quarter, work will focus on harvesting seed from crosses, beginning collection of 2023-24 season yield and fruit quality data on trials with early scions, continuing propagation of trees for future trials, and care of existing trees in trials.  Official release of US SuperSour 4 and US SuperSour 5 is planned for the next quarter.3. Please state budget status (underspend or overspend, and why):This is the first quarter of the second year, and budget is on schedule for spending.  One employee left the program, and a new employee was hired to take on those duties.  The project is fully staffed, and work is progressing as planned. 


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