Our project aims to provide durable long term resistance to Diaprepes using a plant based insecticidal transgene approach. In this quarter, we have cloned all the components necessary for this study. The plant transformation vectors containing the GNA, APA and ASAL genes driven by either the root specific RB7 promoter or the citrus derived C1867 promoter have been constructed. Vectors containing the CpTI gene driven by a SLREO promoter that targets the transgene to the mature root cortex have also been produced. In addition, plant transformation vectors containing the gus gene driven by these root specific promoters have also been produced to demonstrate proof of functionality of the root specific promoters. Transformation experiments to produce genetically modified rootstocks with each of this promoters will be initiated in the next quarter as seeds become available.