Our project aims to provide durable long term resistance to Diaprepes using a plant based insecticidal transgene approach. In this quarter,all the transgenic lines produced have been confirmed for gene integration by conventional PCR and analyzed for gene expression using qPCR. 35% of the lines tested have been determined to be high expressers while the rest were medium to low in expression. Cuttings from all the better performing lines have been made and are being rooted in the mist bed for future challenge with Diaprepes. A number of other potential root specific promoters are being evaluated. Several have been cloned and transformation vectors are being produced. Results from our studies have been presented in the World Congress on In vitro Biology.