1. Please state project objectives and what work was done this quarter to address them: Objectives: 1) Test the efficacy of different injection devices, 2) Determine the most effective formulation of OTC, 3) Determine the best month of injection and most appropriate OTC concentration based on tree size. Five field trials were established to test these objectives. The trials had to be modified from the original proposal to accomodate the requests by the industry collaborators TJ Biotech and Invaio. Trials are as follows:Trial 1: Located in SW Florida (Duda) – 8-year-old Valencia/Carrizo trees. This trial examines two different oxytetracycline (OTC) rates and timing of injection using our established procedures.Trial 2: Located in SW Florida (Graves Bros) – 8-year-old Valencia/Kuharske trees. This trial examines different OTC rates and different volumes in which OTC is delivered. We used a different OTC formulation which we were told is the same as ArborOTC (which we have been using in the past) but marketed under a different name.Trial 3: Located on the east coast (Graves Bros) – 9 year-old Valencia/sour orange trees. This trial is conducted together with TJ Biotech and examines different OTC rates, different OTC formulations, and different injection procedures. The trial compares TJ Biotech’s methodology with ours as well as some combinations of both methodologies.Trial 4: Located on the east coast (Graves Bros) – 4-year-old Valencia/x639 trees. This trial is conducted together with Invaio and examines different OTC rates, timing of injection, and different injection procedures. The trial compares Invaio’s methodology with ours as well as some combinations of both methodologies. Invaio’s OTC formulation is the same as ours. We also included a comparison of scion vs. rootstock trunk injection. Per Invaio request, spray application of OTC (Fireline) is included as one treatment as an additional control. Trial 5: Located on the central ridge (King Ranch) – 4-year-old OLL-8/x639 trees. This trial is conducted together with Invaio and is a repeat of trial 4 (per Invaio request). The trial site was chosen by Invaio.Prior to trial establishment several meetings with TJ Biotech and Invaio took place and numerous emails were exchanged to determine locations, tree types, experimental designs, and objectives of trials 3-5. The resulting trials are the result of compromises between both companies needs and our/industry needs.Baseline measurements for all trials were conducted, leaves were collected prior to injection for CLas detection, and May injections were completed. 2. Please state what work is anticipated for next quarter:We will conduct August injections in the trials where injection timing is one of the objectives. 3. Please state budget status (underspend or overspend, and why):Budget status is as anticipated.