1. Please state project objectives and what work was done this quarter to address them: Objective 1: To compare different rates and annual use patterns of trunk-injected OTC on late-season (Valencia) and early-season (Hamlin) sweet orange tree and their effects on health, yield, and fruit quality over a period of 3 years in a commercial citrus production environment.Obective 2: To compare spring with late summer injections in Valencia orange trees to determine if OTC effects on yield can be enhanced, and the harvest window extended, through timing of injections. Two field trials were established in collaboration with Peace River Packing Company, one with Valencia (Varner grove – trial 1) and one with Hamlin (Kurish south grove – trial 2) trees. Trees in both groves were planted in 2005. Trial 1 was established in a split-plot design. The main plot factor is the season of injection at 2 levels: (i) spring injections; and (ii) late summer/fall injections. The sub-plot factor is the injection rate in combination with different annual use patterns as outlined below: Year 1 Year 2 Year 3 1 utc utc utc 2 150% 0 tbd 3 100% 100% 0 4 100% 0 100% 5 75% 75% 0 6 75% 0 75% 7 50% 50% 0 8 50% 0 50% The trial was established in 16 rows of trees, with the main plot factor arranged by row. Eight replications were used, with each replication consisting of 4 trees. The total number of experimental trees is 512 (8 treatments x 4 trees x 8 reps x 2 times). Spring injections were conducted on June 15 using ReMedium and the Flexinject injectors. The OTC solution was prepared directly in the field to prevent degradation. Trees were injected between 9-12 am. Trial 2 was established in a complete randomized block design using the same rate and annual use pattern as in the Valencia trial. The trial was established in 10 rows, each comprising one block, for 10 replications. Each replicate consist of 3 trees. The total number of experimental trees is 240 (8 treatments x 3 trees x 10 reps). Injections were conducted on June 1 using ReMedium and the Flexinject injectors. The OTC solution was prepared directly in the field to prevent degradation. Trees were injected between 9-12 am. 2. Please state what work is anticipated for next quarter: We will complete the September injections in trial 1. 3. Please state budget status (underspend or overspend, and why):The budget is as expected.