This project continues to make progress. We have grafted the new copies of the LuxI plants and they have grown well. We have collected leaf samples from these plants to test them for the presence of acylated homoserine lactone (AHL). We also have already set up a gas chromatography mass spectrometry (GC-MS) method for the detection of AHL in the LuxI plants and we were able to detect these AHL in spiked leaf samples. Our next step is to test the presence of AHL in LuxI plants. In addition, the LuxI plants are being challenged with Diaphorina citri to determine the effect of expressing of (AHL) on the pathogenicity of Candidatus Liberibacter asiaticus (CLas). Our preliminary observation showed that HLB symptoms are localized in specific areas in the LuxI plants, which means that the presence of AHL prevent the spread of CLas pathogen. In our next step, we will use polymersae chain reaction (PCR) to quantify the level of CLas in CLas-infected LuxI and control plants. This work will be accomplished by December 2017. We look forward to the results of this work hoping that it will provided valuable information to the citrus research community.