Two citrus groves, one – 20 year-old Hamlin sweet orange trees predominately on Swingle rootstock and a second consisting of three year old Hamlin sweet orange trees on Swingle rootstock have received acid injection to selected blocks with and without sulfur applications for fifteen months. Irrigation water was acidified at one of four target water pH (7.5, 6.0, 5.0, and 4.0). A controlled released form of elemental sulfur (Tiger 90) was allied at a rate of 500 pounds per treated acre to plots receiving either acidified irrigation water or control plots receiving irrigation water that was not acidified in December. Significant increases in nutrient concentrations of leaves collected in November 2015 were been found in plots at both sites with reduced water and/or soil pH despite increases in soil pH during the summer months (reported in September 2015 report). The greatest increase in leaf nutrient concentrations were found for Mn, and Zn. These results may indicate increased nutrient uptake from soils with soil solutions below 6.5 and is presumed to be because of lower soil pH levels (5.5 to 6.5). Average water uptake by trees affected with HLB continue to be 20%-25% lower than healthy trees. Water uptake for trees receiving water supplemented with calcium bicarbonate was significantly reduced compared with health trees. Despite higher soil pH, tree root densities were not significantly different for HLB affected trees irrigated with water supplemented with calcium carbonate when compared with healthy trees irrigated without supplemental calcium carbonate. Tree size remain similar for all treatments.