Work continues with a susceptible CLM colony towards estimation of LD50s and LD80s for some commonly used insecticides. Larvae and adults from the susceptible colony are exposed for 48 h to different doses (0; 0.01; 0.03; 0.1; 0.3; 1; 3; 10; 30; 50; 100, 300, 600 and 1000 ppm) of: Actara 25WG, Agri-Mek 0.15EC, Cyazypyr, Danitol, Delegate WG, Dimethoate, Intrepid 2F and Micromite 80WGS. For CLM larvae: Actara (thiamethoxam) LD50=4.18 (CL95: 1.86-8.51) ppm, LD80=524.12 (CL95 209.70-1827) (n = 1047; .2 = 9.54; d.f = 10; Heterogeneity = 0.95); Agri-Mek (abamectin): LD50= 0.314 ppm (CL95: 0.13-0.69), LD80=16.68 ppm (CL95: 6.14-78.56) (n = 1249; .2 = 13.17; d.f = 8; Heterogeneity = 1.65); Cyazypyr (cyantraniliprole): LD50=43.36 ppm (CL95: 15.80-131.55), LD80=49,413 (CL95: 0.19E+07) ppm, (n = 1,196; .2 = 7.64; d.f = 8; Heterogeneity = 0.96); Danitol (zeta-cypermethrin): LD50=381.78 (CL95: 86.33-11,771), LD80= 37, 220 (CL95: 31.70-53761.1) ppm (n = 561; .2 = 5.29; d.f = 9; Heterogeneity = 0.59); Delegate (spinetoram): LD50=2.67 (CL95: 0.87-6.42), LD80= 93.75 (CL95: 33.89-489.58) ppm (n = 938; .2 = 10.69; d.f = 7; Heterogeneity = 1.53); Dimethoate (dimethoate) LD50=1.56 ppm (CL95: 0.15-56.31) and LD80=497.45 ppm (CL95: 22.27-0.37E+09) (n = 546; .2 = 13.07; d.f = 6; Heterogeneity = 2.18); and Micromite (diflubenzuron): LD50=74.30 (CL95: 13.63-285.15), LD80=5,723 ppm (CL95: 994-0.129E+07), (n = 816; .2 = 19.92; d.f = 9; Heterogeneity = 2.21). For adults: Micromite LD50=387.40 (CL95: 143-2817), LD80= 9392 (CL95: 0.192E+07) ppm have been estimated but further replicates are required. Contact toxicity for Intrepid LD50 and LD80 values remain inestimable at the concentrations tested (up to 10 ppm). We will continue tests of higher concentrations. Delegate (spinetoram) LD50=2.84 (CL95: 0.77-73.74), LD80= 183.52 (CL95: 16.47-0.44E+07) ppm (n = 430; .2 = 6.30; d.f = 4; Heterogeneity = 1.58). Additional tests of Actara 25WG, Agri-Mek 0.15EC, Cyazypyr, Danitol, and Dimethoate are slated for the coming weeks. Work also continues on the insect growth regulator, methoxyfenozide (Intrepid). This insecticide acts as an ecdysone agonist, interfering with molting, and exhibiting low contact toxicity as indicated above. Therefore, we have initiated larval bioassays on caged branches infested with CLM in the laboratory. Applications of doses of 0; 0.01; 0.03; 0.1; 0.3; 1; 3; 10; 30; 50; 100, 300, 600 and 1000 ppm are applied when infested leaves contain 3-4th instar larvae. Evaluations of mortality will be conducted 14d after application. We have also initiated work to develop a bioassay method using artificial diet and two diet recipes and are being tested. We are developing a method to collect large numbers of CLM eggs from the established laboratory colony. Eggs will be transferred to cups containing insecticide-spiked artificial diet to determine diagnostic doses for this compound against larval CLM. Field-collected larvae are currently being evaluated for their responses to these insecticides. Although cases of field resistance have thus far not been observed, populations of CLM are currently high in groves in SW Florida and subjected to frequent insecticide applications for control of ACP, CLM and other pests.