The general goal of this project is to rapidly propagate complex citrus rootstock material for field testing. The rootstock materials to be tested will be products of the Citrus Improvement Program at the UF-IFAS-CREC in Lake Alfred. Dr. Jude Grosser has assumed responsibility for completing the project, following the resignation of Dr. Barrett Gruber. Ms. Amy Dubois is the OPS assistant taking care of the trees at the IRREC, she will continue in this roll. The inventory of recovered liners that could make a good field tree are provided below. These will be grown to grafting size, grafted with selected scions (including a dark red grapefruit somaclone N11-15 showing possible tolerance/resistance to HLB) and planted in IR field trials. Additionally, we now plan to include selected new cybrid grapefruit clones (Flame, Ruby somaclone N11-11, and White Marsh) that contain cytoplasm from ‘Meiwa’ kumquat as scions. These new grapefruit clones have shown significantly improved canker tolerance in greenhouse assays as compared to traditional grapefruit clones, and they are not GMO. Having them in the field on improved rootstocks will expedite determining their true canker resistance, as well as their response to HLB. Dr. Ahmad Omar will assist with grafting. Viable cutting inventory: Rootstock # of liners recovered 1. A+HBPxCH+50-7-12-14 44 2. 46×31-00-S10x46x31-00-S11-S5 78 3. Orange 10 x Green 7-11-1 52 4. A+VolkxOrange19-11-5 90 5. A+HBJL2BxOrange14-09-7 71 6. A+HBJL2BxOrange19-09-31 14 7. A+HBJL1-09-14 25 8. A+FDxOrange19-11-11 50 Viable seedling inventory: 1. 46×20-04-S22 86 2. 46×20-04-42 94 3. 46×20-04-48 78 4. 46×20-04-S13 86