Citrus huanglongbing (HLB) is presently the most devastating citrus disease worldwide, and the Florida citrus industry is fighting for its survival against HLB. However, high disease pressure and natural selections of citrus genetic variants provide a golden opportunity for selecting seedlings and/or budsports with greater HLB resistance/tolerance, along with advantage of less time course and no regulatory constraints. This project was built upon a proof of concept on the screening of large numbers of Duncan seedlings via graft inoculation, and the performance observation of 15-25 years old Red Ruby volunteer seedlings (VS) in HLB endemics fields. Twenty of the 300 VS trees were selected, and propagated on sour orange rootstocks in greenhouse. These propagates were evaluated for HLB resistance/tolerance via graft inoculation twice (six months apart) with two different Candidatus Liberibacter asaticus (Las) isolates. Four out of the 20 lines were further selected and propagated on three different rootstocks (sour oranges and US 942) with over 1000 trees. These selections were then planted either in USHLR research farm, Pico’s Farm or the Scott Groves where the VS trees were identified. After almost three years’ evaluation in Pico’s Farm with an extremely high psyllid and HLB disease pressure, the obvious and significant results have been perceived by stakeholders during their field trips. In details, some of the selections showed much lower infection rate (less than 20%) than the control (40%) and poor performers (40-50%) after 26 months evaluation with periodic qPCR assays. It is worth to point out that among the four selections, the best one displayed the lowest disease rate (13.0%) and better growth canopy. Meanwhile, the new plantings (750 trees) in Scott Groves are less than one year old, but they grow well as expected and will be further evaluated. The fruit quality (Brix, sucrose, glucose and fructose, soluble solids, pH, % TA and total ascorbic acid) of the four selected VS trees showed no significant difference from their neighbor maternal trees and commercial grapefruit trees as control both in the original VS trees and their progenies in the new plantings. In other words, these selected VS trees inherited the genetics for good fruit quality when they are propagated on good rootstocks. One such a new selection of rootstocks not only shares the general feature of sour orange, but also yield less size of canopy, which makes it possible for high density of planting. It is well known that it takes at least 5-6 years to evaluate a citrus cultivar for its HLB resistance/tolerance and other major horticulture features in fields. Although the grant terminated in the middle of the project, the selectee and the methods developed for rapid selection and evaluation of citrus variants may serve as bases for developing a new approach to obtain HLB resistant/tolerant cultivar(s) for future citrus industry.