The objective of this study is to determine how different rotation schedules of commonly used insecticides with different modes of action such as dimethoate (acetylcholinesterase inhibitor), imidacloprid and thiamethoxam (acetylcholine receptor), diflubenzuron (inhibitor of chitin biosynthesis), abamectin 3 % + thiamethoxam 13.9 % (chloride channel allosteric modulator and acetylcholine receptor), fenpropathrin (sodium channel modulator), and cyantraniliprole (ryanodine receptor modulator) may impact the level of insecticide resistance Asian citrus psyllid (ACP) populations. An associated goal is to determine if ACP populations can be managed to reduce resistance in those populations where it already exists to a particular insecticide under rotations. Finally, the rotations must be effective in managing existing ACP populations to acceptable grower standards. We have selected two locations where resistance to neonicotinoid insecticides has been demonstrated and is known to exist. At each location, three rotational schemes of insecticides for ACP management will be established in 5 acre plots in Lake Alfred and 4.2 acre plots in Wauchula. The trees are 1-2 years old �Hamlin� trees with a variety of rootstocks. We are collecting adult ACP currently from these locations to determine their baseline insecticide resistance levels compared with a susceptible laboratory population of ACP using a leaf dip assay. Field populations have been collected from the Wauchula site and bioassays are underway currently. We will be collecting psyllids from the Lake Alfred site shortly. We will use commercial formulations of dimethoate, fenpropathrin, imidacloprid and cyantraniliprole to determine baseline resistance levels for these populations. Five to six concentrations of each insecticide will be tested and replicated 5 times. We will begin insecticide applications to fully evaluate our rotation treatments in early April 2019. We will collect samples chosen at random from the central rows for both the Lake Alfred and Wauchula sites. The plots will be sampled weekly beginning in late March 2019. The tap sample method will be used to sample adults. Ten samples will be taken per plot. For eggs and nymphs, 10 randomly selected flush samples will be taken per plots and number of eggs and nymph per flush samples will be counted. When counts of adults, eggs or nymphs in any plot reaches a predetermined threshold, a spray will be applied with the next insecticide in the rotation. Also, we will collect adults from the rotation sites to determine the relative expression of ten CYP4 and six GST genes that are implicated in insecticide resistance in ACP compared with the laboratory susceptable population. Finally, our goal is the development of a more refined method of an effective insecticide resistance management strategy. Our newly developed methods will be have positive impact on suppression of ACP populations by stabilizing or reducing resistance and will be economically viable.