Functional disruption of the NodT outer membrane protein of Candidatus Liberibacter asiaticus for rootstock-mediated resistance to citrus greening using a phloem-directed, single-chain antibody

Functional disruption of the NodT outer membrane protein of Candidatus Liberibacter asiaticus for rootstock-mediated resistance to citrus greening using a phloem-directed, single-chain antibody

Report Date: 04/26/2017
Project: 11-125-424   Year: 2017
Category: Horticultural & Management
Author: Tim McNellis
Sponsor: Citrus Research and Development Foundation

During the period of January, February, and March of 2017, progress was made in planning for HLB resistance testing of the grapefruit lines expressing the FLT-antiNodT fusion protein. Through ongoing discussions mediated by Dr. Catherine Hatcher, Dr. McNellis is in contact with Dr. Johnny Ferrarezi at the Indian River Research and Education Center at Ft. Pierce, and others, to perform no-choice psyllid feeding tests on ‘Duncan’ grapefruit plants expressing the FLT-antiNodT protein. In addition, we are working with Dr. Ed Stover to get some of the FLT-antiNodT plants available at Ft. Pierce to be included in a field test underway at the Pecos farm at Ft. Pierce. Dr. Gottwald’s lab has successfully propagated all eight of the FLT-antiNodT grapefruit lines at Ft. Pierce, with 6-18 plants available for each line, for a total of 75 rooted FLT-antiNodT plants available at Ft. Pierce. During the next reporting period, we plan to apply for permitting to participate in the field test with the FLT-antiNodT lines and initiate the no-choice feeding HLB inoculation test. Dr. McNellis was also invited to participate in the May 22, 2017, Forum on Citrus Breeding and Transformation for HLB resistance hosted by the National Academies of Sciences, Engineering, and Medicine in Irvine, CA, and he plans to attend in person and present at the forum.


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