Generation of non-transgenic HLB-resistant sweet orange varieties using CRISPR-Cas technology

Generation of non-transgenic HLB-resistant sweet orange varieties using CRISPR-Cas technology

Report Date: 11/01/2022
Project: 21-028   Year: 2022
Category: Horticultural & Management
Author: Nian Wang
Sponsor: Citrus Research and Development Foundation

The purpose of this project is to generate non-transgenic HLB resistant Valencia and Hamlin sweet orange plants using CRISPR-Cas technology. Objective 1. Generate non-transgenic HLB resistant/tolerant Valencia and Hamlin sweet orange plants by mutation of HLB susceptibility genes. In total, six putative S genes will be edited. Constructs needed for CRISPR genome editing are being made. Multiple edited lines were generated for ACD2 gene. However, further confirmation demonstrated none of them was biallelic/homozygous mutant. Objective 2. Generate cisgenic genome modified Valencia and Hamlin sweet orange plants by knock-in the gene encoding MaSAMP from Microcitrus. We are optimizing the knock-in method using the CRISPR technology.            


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