1. Please state project objectives and what work was done this quarter to address them: Objective 1: Evaluate currently available registered insecticides in Florida citrus against DRW Establishing laboratory rearing culture of DiaprepesDiaprepes adults were collected from citrus groves in central Florida. Adult weevils were placed into a plexiglass/screen mesh (16″ x 16″ x 16″) cages under greenhouse conditions [27 ºC, with 60% relative humidity, and a 14:10 (light: dark) photoperiod)]. Two to three 1 oz cups filled with water were placed into cages along with citrus leaves as a food source. Wax paper strips (2″ x 3″) were placed in the cages as a substrate for Diaprepes to lay their eggs. After the eggs were laid, the wax paper was removed and placed into Ziploc bags (1gal) with moistened cotton strips. The bags were placed into a plastic container, held at room temperature, and monitored for neonates hatch (7-8 days after egg laying).Toxicity of Exirel (cyanatriliprole) against Diaprepes neonates in soilInsecticide bioassays were initiated with cyantraniliprole. This insecticide is an anthranilic diamide, which is a relatively new class of insect control chemicals that were developed to selectively activate insect ryanodine receptors resulting in uncontrolled release of calcium ion stores causing insect mortality. Cyantraniliprole has been used as an alternative to existing insecticides because of its novel mode of action and acute toxicological profiles. This insecticide has been found to be particularly effective against Asian citrus psyllid and citrus leafminer and therefore it has seen significant use since its introduction into citrus production in ’13-’14. Importantly, this molecule can be delivered with both systemic and foliar formulations making it the only mode of action alternative to neonicotinoids for young tree protection.Initial experiments were conducted to test the effectiveness of formulated cyantraniliprole neonate larvae as a soil application at field label rate (15 oz/acre). Candler sand was sieved using a 6-inch N.B.S. #20 sieve (pore size, 841 M) to remove particulate matter. Approximately 100 g of this soil was air-dried at 40°C for 12hr before adding 9.5 ml of deionized water to achive 12% moisture content. Approximately 5 g of soil was then added to bioassay columns to a depth of 3 cm. Columns were constructed from polystyrene tubes (9.0 cm height, 1.5 cm diameter). This bioassay is based on previous designs employed for evaluating insecticides against Diaprepes. Cyantraniliprole was diluted in SDW at field rate according to the label, and 0.310 ml was pipetted uniformly onto the soil in bioassay columns. Deionized water alone served as the control. Each treatment was replicated four times. Five neonate Diaprepes larvae were scattered on the surface of the soil, and bioassay units were held at 25°C. There were 12 replicate bioassay chambers conducted per replicate. After 48hr, the number of living and dead larvae recovered in the containment cell at the base of the bioassay column was counted using a dissecting microscope. Thus far, we have only been able to evaluate the field label rate of cyantraniliprole. At the field label rate, we found 100% mortality with cyantraniliprole treatment at label rate, while only 10% of larvae died at the end of the bioassay period in the control. We are currently continuing with the dosage series to determine both the LD50 value and to determine a concentration range over which he chemical is effective. Furthermore, now that the culture is established and bioassay has been developed, we will be moving forward with the other chemicals that require evaluation. Our upcoming assays will evaluate flupyrandifurone (Sivanto), thiamethoxam (Olatinum), and imdacloprid (Various products).Objective 2. Determine the source of DRW infestation and how their dispersal affects management decisions.Emergence and adult interception traps have been placed in two sites since the fall of 2022. To date, only a few adult Diaprepes have been collected from one site. We are currently expanding to a third site and adding traps into the natural areas surrounding the production fields. This low population is not unexpected as Diaprepes are generally more active from mid May though June. 2. Please state what work is anticipated for next quarter:Insecticide screening and trapping will continue to explain within field population density variations.Once Diaprepes begin to emerge, we will begin marking and capturing them in the field to better understand their dispersal. 3. Please state budget status (underspend or overspend, and why): On track