High-throughput screen of seedlings for resistance to citrus greening based on optical sensing

High-throughput screen of seedlings for resistance to citrus greening based on optical sensing

Report Date: 11/02/2014
Project: 880   Year: 2014
Category: Other
Author: William Gurley
Sponsor: Citrus Research and Development Foundation

Psyllid-inoculation of citrus seedlings: Sweet orange seedlings have been growing at the CREC (psyllid growth room of Dr. William Dawson) for 7 weeks. During that period the seedlings were exposed to feeding by HLB-positive psyllids. These plants are scheduled for harvesting after week 8. Preparations are underway to catalogue every leaf on each plant using three emission filters (500nm, 690nm and 730nm) using three excitation wavelengths (UV 364nm, Blue 470nm, Green 527nm) in addition to polarization images at 594nm). Results from image acquisition and PCR results will be included in the next progress report.


Your browser does not support pdfs, click here to download the file.