During the first quarter of funding, Core Citrus Transformation Facility (CCTF) continued to process the orders for transgenic Citrus material. Demand for genetically transformed citrus plants stayed high resulting in influx of new orders listed here by names of genes of interest or plasmids: p6; p33; p7; p10; pMOG8000; pAS7; pAS13*; pNAC1; pMKK7; pMOD1; and pSucNPR1. The work also continued on the old orders that were previously partially completed. Considering that transgenic Duncan plants carrying NPR1 gene exhibited significant resistance to Citrus canker, CCTF received order for production of commercially important Flame grapefruit cultivar transformed with the same NPR1 gene (order completed-NPR1 gene: 10 plants). A gene thought to be superior to NPR1 (so-called superNPR1) was introduced into Duncan grapefruit (superNPR1 gene: 12 plants). Introduction of NPR1 into Hamlin orange cultivar and superNPR1 into Flame is half completed. Work on the order pAS7 that is associated with HLB tolerance/resistance is half done (5 Duncan plants). Order that included use of pLC plasmid is also completed (8 Hamlin orange plants). Ten Mexican limes transformed with gene in pHK plasmid were produced, but satisfaction of this order will include production of more plants per client’s request. Five plants of Mexican lime transformed with p33 gene were also produced. CCTF produced more plants for the old orders: N1* gene: 3 Duncan; C5*: 3 Duncan; CL1 gene: 2 Valencia, 3 Duncan; CL2 gene: 1 Duncan; PiTA gene: 1 Valencia; CIT108p: 1 Flame. About thirty more soil-adapted plants will be submitted to the PCR testing as a secondary proof of their transgenicity before delivery to clients. Some of the funds from the grant were used to hire additional help through the summer resulting in seasonal increase of CCTF capabilities. Please be informed that the person directly managing the CCTF (and co-PI) is Dr. Vladimir Orbovic.