As previously reported, trees were steam-treated as described in our research proposal during July 2015. Adults and nymphs were enclosed in mesh sleeves on trees for acquisition feeding approximately 5 weeks following treatments. Following acquisition feeding, insect and leaf samples were collected (45 d post-treatment) from trees and taken to the lab for subsequent nucleic acid extraction and analysis. Acquisition feeding assays were repeated approximately two months later, with samples collections beginning 114 d post-treatment. Nymphs were collected from plants after adult emergence, until no psyllids remained in the mesh sleeves. The titer of Candidatus Liberibacter asiaticus (CLas) in trees receiving steam treatment did not significantly differ from untreated trees on days 0, 45, or 114 post-treatment (p = 0.99, 0.11, and 0.81, respectively; Tukey’s Honestly Significant Difference (HSD) test); however CLas titers in treated and untreated trees were lower at 45 d post treatment as compared to days 0 and 114 post-treatment. This is likely due to naturally-occurring seasonal decreases in CLas titers. CLas titers were significantly higher in steam-treated trees than untreated trees on day 0 as compared to day 45. CLas acquisition by adult psyllids enclosed on trees receiving thermal treatments did not differ significantly from acquisition by adult psyllids on untreated trees. Samples from CLas acquisition feeding assays with psyllid nymphs are still being processed. Based on these results, which indicated the thermal treatments applied during July 2015 did not reduce plant CLas titer or psyllid acquisition, a second thermal treatment was applied during late November 2015. In early January 2016, adults and nymphs were enclosed in mesh sleeves on trees for acquisition feeding approximately 5 weeks following treatments. Insect and leaf samples were collected after 10d of acquisition feeding or upon adult emergence to assess adult and nymph acquisition, respectively. In addition, we have initiated a complementary laboratory study to evaluate the effect of thermal therapy on acquisition of CLas under controlled conditions. Two year old Valencia trees were inoculated with CLas by enclosing plants with CLas-infected psyllids for two weeks. Currently, plants are being held in a secure, insect-free greenhouse until they are determined to be positive for CLas. At that time, a controlled environmental chamber will be used to apply heat treatments to trees for use in subsequent acquisition experiments