Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), adults were collected from eight citrus groves across central Florida and the level of insecticide resistance to ten insecticides was measured by using a bottle bioassay. The gene expression of five cytochrome P450 CYP4 (CYP4C67, CYP4DA1, CYP4DB1, CYP4G70 and CYP4C68) and three glutathione S-transferase (GSTD1, GSTE2 and GSTE1) genes was characterized in seven field populations of ACP and compared with the laboratory population. Finally, we reared four neonicotinoid insecticide resistant field populations in the laboratory and observed susceptibility changes to insecticides without exposure to insecticides over multiple generations. The eight field populations of ACP adults showed no and very low levels of resistance (RR = 1 and 2-10) to dimethoate, chlorprifos, carbaryl, fenpropathrin, bifenthrin, flupyradifurone, and spinetoram. Very low to low resistance was found to imidacloprid and cyantraniliprole (RR = 2-10 and RR = 10-20). Moderate to high resistance was found for thiamethoxam (RR = 20-50 and RR = 50-100). The CYP4G70 and CYP4C68 genes were expressed at a higher level in field populations as compared with the laboratory population. Also, three separate field populations exhibited higher expression of all target genes compared to the laboratory population. For imidacloprid and thiamethoxam, there was a decline in susceptibility by 6.62-and 6.42-fold, respectively, compared to the initial results. These results indicate that the insecticide resistance may reverse in the field if insecticide selection pressure is removed from the spray schedule or with use of a rotational scheme with insecticides of different modes of action. Also the results support use of a survey program combined with effective rotation for integrated insecticide resistance management of ACP where huanglongbing (HLB) management includes vector suppression with insecticides. Importantly, this investigation verified that insecticide resistance in the Asian citrus psyllid carries a significant fitness cost for resistant populations. Thus, in the absence of selection for 5-6 months, psyllid populations return to normal levels of susceptibility. Our investigation demonstrated the value of implementing MOA rotation to manage resistance for ACP, and shows that resistance can be managed effectively by rotating 5 modes of action. These results may have been even more marked if conducted on a larger scale to prevent the local population from inbreeding between treatment applications. Rotation of insecticide modes of action does prevent or delay onset of resistance in ACP populations, and the effects of even relatively short duration selection pressure can be observed even under extreme circumstances where inbreeding with non-selected psyllids is possible. Our results indicate that even brief failures to rotate modes of action during ACP management with insecticides in Florida citrus production may establish localized populations of insecticide resistant ACP.