In this quarter, the propagated transgenic lines have been budded up with non-transgenic HLB free Valencia budwood. It has been observed that the bud take frequency is higher on cutting made from juvenile transgenic lines when compared to the mature lines. Seed source trees, budded onto US802 rootstock have been produced for field evaluation and are being sized up. Transgenic NPR1 lines that have been observed to be tolerant to HLB under field conditions are also being propagated to use as interstocks in this study. ELISA protocols are being developed to rapidly test for transgene products in the fruit and juice.A USDA-APHIS permit has been applied for evaluation of these transgenic lines.