In this quarter, several transgenic rootstocks expressing the AtNPR1 transgene were inoculated with HLB infected Valencia budsticks (November). Most of these were repeats of ones that had failed from the september graftings. As with pervious attempts, the Ct values of the infected budsticks were evaluated prior to stick grafting. Ct values ranged from 24.1 to 27 in the repeat budded set. The objective behind this study was to understand the effect of the transgenic rootstock on HLB. The budsticks have been slow to flush due to the winter. qPCR was performed on both transgenic and control roots as well as leaves but no HLB has been detected so far. it is estimated that it will be several more months before the inoculum can be detected. Transgenic rootstocks budded with non-transgenic valencia scion have also been prepared for planting in the field since we expect the field trial will provide the most conclusive data.