All transgenic rootstocks (Swingle and Carrizo) expressing the AtNPR1 gene, developed using mature tissue transformation and juvenile transformation have been evaluated using qPCR. Several of the promising lines have also been evaluated for the trans-protein production using western blotting techniques. Cuttings of several lines have been propagated and budded with non-transgenic ‘Valencia’ sweet orange. Many others are currently being propagated in the mist bed. To produce seed source trees, trees are being produced by budding onto US802 rootstock. These will be planted in a USDA-APHIS approved field site when ready. Transgenic rootstocks expressing other potent plant derived transgene(s) are also being produced using the mature tissue transformation method.�