Work on this project was already in progress when funding was released in August 2011. Objectives include: 1) scale up production of Tamarixia radiata to levels that will allow better assessment of the potential impact an augmentation program may have on the ACP population and ultimately the spread of HLB, 2) use genetic techniques to identify parasitoids recovered from the field to demonstrate establishment and effectiveness of released strains, and 3) pass on rearing technology to both private and public sectors to encourage production and an adequate supply of these parasitoids into the future. The colony at SWFREC of the previously established strain of T. radiata produced 53,398 wasps this quarter, increasing the 2011 total to 191,570. These wasps were used to maintain the colony and release in treated and untreated plots of commercial experimental blocks in Collier, Lee and Hendry counties. This quarter, 37,300 wasps were released, increasing the number released in 2011 to 124,970. Field releases this quarter of 20,600, 1,700, and 600 wasps from Pakistan, South China and North Vietnam colonies established at DPI, Gainesville join a total of 58,700, 4,185, and 4,700, wasps released this year. Wasps from the Pakistani colony continue to be released at the SWFREC, while wasps from south China and north Vietnam were released in commercial groves in Glades and Charlotte Counties, the latter being organic. Incidence of parasitism based on adult emergence from feral nymphs in the release block at SWFREC (Collier county) averaged 49% (N=565) and 45% (N=93) in July and August, respectively, compared to non release blocks averaging 3% (N=565) and 25% (N=327) in July and August, respectively. Parasitism of feral nymphs collected in August from a release block of a neighboring commercial grove averaged 3% (N = 175). Parasitism of nymphs recovered on sentinel plants placed in the release blocks in August averaged 98% (N=102) at SWFREC and 40% (N=5) in the commercial grove. No nymphs were recovered on sentinel plants placed in the non release blocks at SWFREC but 98% (N=174) were parasitized on sentinel plants placed in the commercial grove, probably indicating movement from the release block. In two sets of sentinel plants placed in September 35-100% (N=31-12) were parasitized at SWFREC and 13-97% (N=13-36) were parasitized in the commercial grove in release blocks. In non release blocks 13-65% (N=23-52) were parasitized at SWFREC and 38-42% (N=89-141) at commercial grove. Feral nymphs were also collected during August and September from experimental groves in Lee and Hendry counties, but less than 1% parasitism was observed. In general feral populations of psyllids were very low in the commercial groves during the report period. The recovered parasitoids are preserved in 95% ETOH and sent to Dr. Evan Braswell of USDA APHIS, Edinburg, TX, to identify genetic origins of these parasitoids using molecular markers. We also initiated the biparental colony of Diaphoencyrtus aligarhensis from Pakistan for release and evaluation at SWFREC. Addionally, we also visited Orange Co. to discuss their production and release system and some collaborative experiments involving release and evaluation of Tamarixia radiata.