The objective of this research project is to investigate and develop a potential non-phytotoxic, environmentally friendly film-forming ACP repellent solution for preventing HLB infection. In the last reporting period, two new organo-silica composite film (OSCF) materials, OS-SG 11 and 12 were synthesized based on the feedback from previous OSCF ACP infection studies. In this reporting period, we designed and developed a new OSCF material (OS-SG 13) using EPA approved materials which is expected to form a film with high roughness after spray application. Safety analysis of OS-SG 11, 12 and 13 were conducted using Tomato sp as a model plant. Phytotoxicity studies were conducted in a Panasonic Environmental Test Chamber (Model MLR- 352H) to control light intensity, humidity and temperature cycling to simulate summer conditions (85% RH, 34oC). All OSCF materials were shown to be safe at the applied rates. The composition and interactions between the components were confirmed using Fourier transform infrared spectroscopy and Nuclear Magnetic Resonance. The morphology of OS-SG 11, 12 and 13 were observed using Scanning Electron Microscopy (SEM) and the elemental confirmation of the OS-SG 11, 12 and 13 were confirmed using Energy Dispersive Spectroscopy. SEM analysis revealed OSCF materials formed a film with high surface roughness. The rainfastness of OS-SG 11, 12 and 13 were studied using rainfall stimulation assay. Tomato sp (model plant) from Home Depot was used for the study. Plants were grouped into 4 groups (0, 1, 2 and 3 rainfalls) and further divided into triplicate. Using a known flow rate, each rainfall was estimated to have produced 4.3 gallons of water per rainfall. Plants were allowed to fully dry between rainfalls. After all rainfalls, all leaves were collected and metals were removed and analyzed for OSCF presence using Atomic Absorption Spectroscopy. Metal analysis of OS-SG 11 revealed that 77% of the composite was left on the surface after 1 rainfall and this was reduced to 51% after 3 rainfalls. This information will be used to help develop proper application rates for OSCF materials. The morphology of OSCF materials after application to leaf surface and film thickness was studied using Atomic Force Microscopy. OS-SG 11, 12 and 13 were delivered to UF CREC for ACP trials.