This project evaluates young tree protection from ACP/HLB using approaches such as ground cover, insecticides, and irrigation management at three locations 1) Southwest Florida Research and Education Center (SWFREC), Immokalee, FL, 2) Citrus Research and Education Center (CREC), Lake Alfred, FL, and 3) Florida Research Center for Agricultural Sustainability, Vero Beach, FL. In this quarter treatments of 1) soil-applied neonicotinoids interspersed with sprays of a different mode of action insecticides on a calendar basis, and 2) rotation of insecticide modes of action sprayed twice on each major flush were implemented to the trees on UV reflective and bare ground. The irrigation deficit treatments to manage flush were also functional at the Immokalee and Lake Alfred locations to trees on UV reflective mulch and bare ground to synchronize flush to target spray applications on major flushes. All the trees were drip-irrigated with two emitters. A separate irrigation treatment using a microsprinkler was also evaluated at the Lake Alfred location.Sampling was conducted to monitor psyllid populations and flush abundance at all three experimental sites. Significant effects of ground cover on psyllid populations were observed at all three locations. At Immokalee, a reduction of 61% in the adult ACP numbers in the plants on mulch compared to bare ground was observed with an average of 0.20 and 0.51 adults per tap sample, respectively. The same level of reduction (61%) was observed at Vero Beach, with an average of 0.07 and 0.18 adults per tap sample on mulch and bare ground, respectively. An overall reduction of 69% using all data on adults was observed at Lake Alfred. At Immokalee, flush infestation with psyllid immatures averaged 22% on mulch and 36% on bare ground, a reduction of 39% on mulch. An average of 5% shoots on mulch and 9% on bare ground were infested at Vero Beach, a reduction of 44% on mulch.Data from April 2021 showed higher soil moisture averages from mulch treatment at all layers (8, 15 and, 45 cm) compared with bare ground. However, these differences were masked by rains in summer 2021. Soil analysis from Immokalee location showed that except for Mg, K, and B, all the other nutrient concentrations were higher in the mulch plots suggesting better nutrient distribution within the root zone and minimal leaching threat. The flush count was impacted by the ground cover treatment (mulch vs. bare) and the date of sampling. On average, more flush was observed on mulched trees than on unmulched trees. The trees on irrigation deficit treatments produced less flush compared to those on the full or conventional irrigation treatment and that trend persisted on the mulch or bare ground.Trees on the UV reflective mulch showed a significant difference in growth. At Vero Beach, compared with last measurements in March, an increase in the growth of rootstock averaged 20.9 mm and 16.8 mm on mulch and bare ground, respectively, whereas scion growth averaged 13.5 and 11.6 mm, respectively. Similar effects on tree health were observed at other locations. Overall, tree canopy density appears to be greater on mulched than bare ground trees at all locations and went up by 40% at the Lake Alfred and 30% at Immokalee. A detailed article Implementing UV reflective mulch and flush timed sprays for managing Asian citrus psyllid was prepared and submitted for publication in Citrus Industry magazine. We are continuing measurements on multiple variables relating to psyllid, HLB and tree health from all locations and hope to better understand the impact of mulch and flush treatments on tree growth and yield in the coming years. The start of this project was delayed significantly due to the logistics involved in setting up the trials in three regions. Therefore, we will need one more year after the end date of December 2021 for the successful completion of this project.