This project is focused on young tree protection from HLB through reduction in populations of vector ACP and irrigation management for improved tree health. Metalized polyethylene mulch and irrigation management can alleviate the problem by repelling ACP and controlling flush cycles to improve ACP spray timing and efficiency. During this quarter we were able to plant at the Gulf region location. The orange trees planted at the Immokalee location were Valencia on Swingle rootstock. The four treatments to be applied to these trees include 1) soil applied neonicotinoids interspersed with sprays of different mode of action on a calendar basis to trees on mulch, 2) rotation of insecticide modes of action sprayed twice on each major flush to trees on mulch, 3) soil applied neonicotinoids interspersed with sprays of different mode of action on a calendar basis to trees on bare ground, 4) rotation of insecticide modes of action sprayed twice on each major flush to trees on bare ground. Trees were planted late due to logistics and therefore it was not possible to establish a drought period to manage flush for the treatments 2 and 4, which includes spraying on flush. Therefore, all plots will be allowed to establish a first spring flush and then the drought treatment will be applied to synchronize flush and evaluate spray applications. Soil application of Admire Pro were made in treatments 1 and 3. Sprays of Delegate were made to trees in treatments 2 and 4 in September and Transform to all trees in October. So far, 3 ACP detected throughout the experiment one each in treatments 2, 3 and 4. We covered twenty-four trees with tree defenders, and these will be compared with unprotected trees for ACP and HLB incidence and tree health. Experiment at the Ridge location was planted during the previous quarter. In September, 2 adult ACP and 2 infested flush were observed on a plant in treatment 4.