This project evaluates young tree protection from ACP/HLB using approaches to integrate ground cover, insecticides, and irrigation management at three locations 1) Southwest Florida Research and Education Center (SWFREC), Immokalee, FL, 2) Citrus Research and Education Center (CREC), Lake Alfred, FL, and 3) Florida Research Center for Agricultural Sustainability, Vero Beach, FL. Treatments of 1) soil-applied neonicotinoids interspersed with sprays of a different mode of action insecticides on a calendar basis, and 2) rotation of insecticide modes of action sprayed twice on each major flush are being implemented to the trees on UV reflective and bare ground. The irrigation deficit treatments are also functional at the SWFREC and CREC locations to trees on UV reflective and bare ground to synchronize flush to target spray applications on major flushes. Sampling was conducted to monitor flush abundance and psyllid populations at three experimental sites. At the Vero Beach location, significantly more flush was observed in the trees on mulch compared to the trees on bare ground averaging 4.09 and 3.47 per plant, respectively. The diameter of the trunk of the rootstock and scion of the trees on the mulch was significantly more than the trees on the bare ground. An average rootstock diameter of 33.79 mm on the mulch and 28.20 mm on the bare ground was observed. Scion diameter averaged 24.04 mm on the mulch and 21.12 mm on the bare ground. At the CREC location, on average, there was also significantly more flush observed on mulched trees than on bare ground trees. Analysis of data from September 2020 to March 2021 showed significantly fewer adults on trees with mulch than with no mulch. Trees with insecticides applied based on a calendar schedule also had fewer adult ACP than those that experienced insecticide applications based on flush. ACP adult abundance was weakly but positively dependent on flush abundance. Peaks of adult counts occurred between March to June as well as September to October in 2020 and this is where treatment differences were starkest. Significantly fewer eggs and nymphs were observed on the trees with mulch than those on the bare ground. At SWFREC, trends on flush abundance across treatments and ACP response to the treatments were similar to other two locations. During this quarter, the mulched and non-mulched treatments soil moisture contents varied between 0.11±0.02 m3 m-3 and 0.10±0.01 m3 m-3 showing not much difference in water storage with the root zone. Overall, canopy density appears to be greater on mulched than non-mulched trees. In the next quarter, we will do leaf and soil nutrient analysis and canopy size determinations to see changes if any in the treatment effects. At SWFREC, several experiments were conducted using the remote-controlled and manual ground-penetrating radar (GPR) operation to evaluate the effort needed to perform the GPR scanning, measure the required time to complete the scanning process, evaluate precision and accuracy of the scanning circles, and determine if the remote-controlled system can perform better for non-invasive root detection than the manual process. The scan line data collected from both the remote-controlled and manual process were compared after processing the data using an auto root detection software (Tree Radar Inc., Maryland, USA.) The results of root detection for both processes were almost the same, and there were no significant differences between them. The depth of the detected roots in the remote-controlled process was same as of the manual process. These experiments show that the remote-controlled process can reduce the required application time by 3 times when compared to a manual process. It can also reduce the human effort required and increase the precision of the data collection process. A Qualtrics survey to obtain growers input on the reflective mulch technology was developed and distributed through several channels.