Preventing young trees from psyllids and infection with CLas through use of protective netting

Preventing young trees from psyllids and infection with CLas through use of protective netting

Report Date: 06/12/2020
Project: 18-032C   Year: 2020
Category: ACP Vector
Author: FERNANDO ALFEREZ
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:  Work in this quarter has been affected by the restrictions due to the COVID-19 situation. However, we have accomplished most of the work that was scheduled. Objective 1. Assessing tree growth and absence of psyllids and HLB disease symptoms (including CLas bacteria titer) under protective covering. We have continued monitoring trunk diameter and canopy area. Trunk diameters in IPC covered trees are starting to show bigger diameters with statistical significance. All IPC trees are still HLB-negative and show less incidence in canker. We have continued monitoring the incidence of pests and psyllids. We have shown that leaf drop is seasonally affected but under IPC is significantly less. Objective 2. Assessment of alternative netting approaches involved in ‘targeted’, ‘alternated’ or ‘patterned’ setup of IPC in groves for more cost-effective protection. After planting the new 700 trees last quarter in an alternated pattern we are monitoring CLas in trees adjacent to the IPC-covered trees. New plantings are expected to study the effect of patterned IPC disposition in the grove. Objective 3. Monitoring the transition from vegetative to reproductive stage in the covered trees as compared to the uncovered . Parallel experiments at SWFREC, Hendry Co, and Central Florida have been performed using Bingo , Early Pride, and Tango trees to determine the ability of these varieties to set fruit in the absence of pollinators. We will be collecting data on these trials starting next quarter. Objective 4. Comparing IPC with CUPS-like systems. We are monitoring the effect of deficit irrigation in all the varieties and we have seen that we can control blooming: we see more bloom inside CUPS and IPCs after applying deficit irrigation. Outreach activities  performed this quarter:-Tango Grower meeting by zoom, May 26. 40 growers attended.-Bingo Grower meeting by zoom, May 19, 26 growers attended.-Bingo Grower Working Group. Kickstart meeting at CREC, February 27.-In ServiceTraining for Extension Agents online: “Horticultural practices: IPCs”. 2. Please state what work is anticipated for next quarter: Objective 1. We will continue monitoring parameters described in the first section. Also we will continue chlorophyll and starch analysis. We will remove the covers in the trees from the first experiment to monitor HLB start and incidence rate.Objective 2. New plantings will be adopting the patterned IPC configuration.Objective 3. We will assess fruit set success after June drop and compare outside-grown trees with IPC- and CUPS-grown trees.Objective 4. We will continue to monitor growth and physiological parameters for comparison in CUPS, IPC, and outside-grown trees after deficit irrigation experiments. -Presentation at Citrus Expo: “IPCs. New data on tree performance and lessons learned”. Fort Myers, August 2020. Publications:-Individual Protective Covers’ by Alferez, F, Gaire, S., Albrecht, U., Batuman, O., Qureshi, J., Zekri, M., to be submitted to EDIS.-Gaire, S., Albrecht, U., Batuman, O., Qureshi, J., Zekri, M., Alferez, F. 2020. Horticultural performance of citrus trees grown under Individual Protective Covers (IPCs). To be submitted to Plants, MDPI      3. Please state budget status (underspend or overspend, and why): So far, we have spent about 33% of the budget. We are in the middle of the project duration, so we have underspend so far. This is due to two main things: 1) Budgeted meetings (national and international) and publications have not occurred yet, and 2) some expensive reactives for analysis have not been purchased as sampling has not been completed yet. Some delays in spending due to COVID have occurred as well.Budgeted amounts for salaries and student stipend and tuition are being spent as predicted.   


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