Propagation of Rootstock Tree Production in Greenhouses by Seed, Stem Cuttings and Tissue Culture to Accelerate Budded Tree Production for Outplanting

Propagation of Rootstock Tree Production in Greenhouses by Seed, Stem Cuttings and Tissue Culture to Accelerate Budded Tree Production for Outplanting

Report Date: 08/07/2016
Project: 940C   Year: 2016
Category: Other
Author: Richard Beeson
Sponsor: Citrus Research and Development Foundation

Tissue culture. Tissue culture plants started on 3-09-2016 were subjected to the same 4 durations of relative humidity (RH; 2,3,4 and 5 days) as previously. Plants were harvested on 28 April for root and shoot growth. Citrus cultivars consisted of C-35, C-54 and sour orange. Dry mass measurements are continuing. The last set of tissue culture plants for hardening were started on 25 May 2016. These will be harvested on 13 July. This set includes Kuharske, Swingle, C-35, sour orange and US 812. For the most part, survival rates have remained high on most cultivars, with perhaps more growth under the shorter hardening-off periods. Stem cuttings. Cuttings of Kurharske, C-54, C-35, US 812 and sour orange were treated with 4000 ppm Dip&Gro on 8 April, 28 April, 20 May and 10 June. This completed 1 year of initiating the rooting of cutting every 3 weeks. Concurrently stem cutting stuck previously were harvested for set #13 on April 6, with the remaining harvested on May 3, May 20, June 10 and the last replication harvested on June 30. With the harvest of the April 8 cuttings, root development and growth was similar to that early summer 2015, and was continued until the end of the experimental year. All plants were dried at 70C. Measurement of root growth and dry mass is continuing. On 25 March, all available cuttings of the HLB resistant trees in Citra were taken and brought to the citrus propagation bay at MREC Apopka. These were treated with the 4000 ppm auxin and stuck in seedling trays. On 25 April cuttings were selectively harvested from MREC block and stuck. Total HLB resistant cuttings stuck this spring were UFR7 � 216, UFR9 � 473, UFR10 � 153, UFR11 � 374 and UFR12 � 143. Additional cuttings will be propagated as required by Dr. Grosser.


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